Journal: European journal of immunology

Article Title: Vaccination with myelin oligodendrocyte glycoprotein adsorbed to alum effectively protects DBA/1 mice from experimental autoimmune encephalomyelitis.

doi: 10.1002/eji.200323772

Figure Lengend Snippet: Fig. 3. Immunization with rMOG-alum prevents infiltration of CD4+ T cells into the CNS upon subsequent injection of rMOG-CFA. Lymphocytes were isolated from the CNS of mice 12 days after injection of rMOG-CFA. The mice had either been pre-treated with rMOG-alum (open squares) (n=4) or with alum alone (filled squares) (n=4) 7 days prior to rMOG-CFA injection. The cells were stained with antibodies specific for CD4 and CD25. The graph depicts the percent- age of cells within the lymphocyte gate that display the markers. The number of CD4+ T cells, especially activated cells, is significantly reduced in rMOG-alum-treated mice (Mann-Whitney test, p X 0.05). Fig. 4. Sera from mice immunized with rMOG-alum i.p. prior to injection of rMOG-CFA display a different antibody profile compared with controls, as determined by isotype ELISA. (a) Pre-treatment with rMOG-alum i.p. (open histograms) skews the antibody response to be less type-1-like, with more IgG1 (p X 0.005) and less IgG2a (p X 0.005) than in controls (filled histograms). There was no significant difference in total IgG (IgG tot) or IgG2b titters, and no IgE was detected. (b) Pre- treatment with rMOG-alum s.c. skews the antibody response in a similar way. Differences between groups were tested with the Mann-Whitney test. The results are represen- tative of three experiments.

Article Snippet: ELISA of serum samples collected on day 16 was conducted as described previously [21] using biotinylated goat anti-mouse IgG (Dako, Denmark) for total IgG and rat antimouse IgG1, IgG2a or IgG2b (all of these antibodies from Serotec, Oxford, GB) for isotype determination.

Techniques: Injection, Isolation, Staining, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay